Hyperspectral detection in flow cytometry is a powerful tool for over ten multi-color phenotypic analysis without compensation. There are many operating flow cytometers in the marketplace with only 4 or 5 color performance. An objective of this work is to provide a universal hyperspectral detection system to analyze fluorescence signals from a variety of flow cytometers. Using our prototype we were able to evaluate a wide wavelength range (340-800nm) with 42channels and 16 bit resolution.

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